ABSTRACT
<p><b>OBJECTIVE</b>The aim of this study was to investigate the mRNA expression levels of human leukocyte antigen Class I at different progressive stages of human oral squamous cell carcinomas.</p><p><b>METHODS</b>The expression of mRNA of human leukocyte antigen--B/C was detected in 23 primary tumors, 10 metastatic focuses and 11 histological normal oral epithelia using in situ hybridization method with a digoxigenin--labeled DNA probe. The probe was human leukocyte antigen--B/C locus specific.</p><p><b>RESULTS</b>The hybridization signals were present in the cytoplasm of either normal epithelia or tumor cells. The integrated optical density values of the hybridization signals were detected with the aid of an image analysis system. The results showed that the average integrated optical density values of the primary tumors were statistically lower than the normal oral epithelia (P < 0.05), but there was no significant difference between metastatic tumors and the primary tumors or the normal epithelia. The integrated optical density values measured in the metastatic tumors also did not show statistically differences compared with the primary tumors of the same patients.</p><p><b>CONCLUSION</b>Impaired regulation of human leukocyte antigen--B/C transcription could occur but might not be directly associated with metastasis of oral squamous cell carcinomas.</p>
Subject(s)
Humans , Carcinoma, Squamous Cell , Genetics , Allergy and Immunology , HLA-B Antigens , Genetics , HLA-C Antigens , Genetics , Histocompatibility Antigens Class I , Genetics , In Situ Hybridization , Mouth Neoplasms , Genetics , Allergy and Immunology , RNA, Messenger , Genetics , Transcription, GeneticABSTRACT
<p><b>OBJECTIVE</b>The purpose of this study was to investigate the transcription and expression levels of human leukocyte antigen-DR at different stages of oral squamous cell carcinomas.</p><p><b>METHODS</b>The specific monoclonal antibody and beta-locus specific oligonucleotide probes of human leukocyte antigen-DR were employed in this study. A total of 32 primary oral squamous cell carcinomas, 15 metastatic focuses and 26 histologically normal oral epithelia, were detected for the presence of the human leukocyte antigen-DR molecule by using immunohistochemistry and in situ hybridization.</p><p><b>RESULTS</b>The human leukocyte antigen-DR expression of the primary focuses was significantly higher than that of the normal epithelia (P < 0.05), but their expression did not show statistically difference between the metastatic focuses and the normal epithelia. The immunohistochemistric results were identical with those of in situ hybridization.</p><p><b>CONCLUSION</b>The abnormal higher expression of the HLA-DR is a common character of primary oral squamous cell carcinomas, but it may be not relevant to the metastasis of tumors.</p>
Subject(s)
Humans , Carcinoma, Squamous Cell , Genetics , Allergy and Immunology , HLA-DR Antigens , Genetics , Immunohistochemistry , In Situ Hybridization , Mouth Mucosa , Allergy and Immunology , Mouth Neoplasms , Genetics , Allergy and ImmunologyABSTRACT
Objective To make the clinical evaluation of Targis/Vectris restorations. Methods 337 cases with Targis/Vectris restorations were rechecked 12～50 months after cementation respectively. The restorations were evaluated clinically according to the USPHS standard. Results The changes of Targis/Vectris restorations in colour match, boundary line, wear and tear, marginal adaptation and surface grain were not obvious within 12～50 months. The secondary caries were not found. The successful rate of the restorations was 95.4 %.Conclusion The clinical prosthetic results of Targis/Vectris restorations are reliable.
ABSTRACT
Objective: To observe HLA DR expression in different s tages of oral squamous cell carcinoma (OSCC) and to study the clinic significan ce of the abnormal HLA DR expression in primary OSCC. Method: HLA DR expression was detected by immunohistochemistry method in 26 cas es of histologically normal oral epithelia, 8 leukoplakia, 32 primary focuses an d 12 metastasis focuses of OSCC. Results: HLA DR express ion in primary OSCC focuses was significantly higher than that in normal epithel ium( P 0.05). Conclusion: Although HLA DR expression is frequently obs erved in the development of OSCCs, it can not be regarded as one of the indepen dent prognosis factors.